| seiyuu | role | episode |
| Suzukaze Mayo | Himura Kenshin | [debut] |
| Fujitani Miki | Kamiya Kaoru | [debut] |
| Ueda Yuuji | Sagara Sanosuke | |
| Tominaga Miina | Myoujin Yahiko | |
| Shiratori Yuri | Tsubame | |
| Namiki Noriko | Suzume | |
| Yuasa Kaori | Ayame | |
| Sakurai Tomo | Makimachi Misao | |
| Hidaka Noriko | Seta Soujirou | |
| Ikeda Shuuichi | Hiko Seijuurou | |
| Doi Mika | Megumi | |
| Suzuoki Hirotaka | Saitou Hajime | |
| Ohtsuka Akio | Kurogasa | |
| Tanaka Mayumi | Tsukayama Yutarou | |
| Suzuki Masami | Ebisu Marimo | ep 17 |
| Honda Chieko | Arai Azusa | ep 39-40 |
| Nishihara Kumiko | Mikan | ep 48 |
| Kawata Taeko | Goro | ep 48 |
| Akado Mayumi | Taisuke | ep 48 |
| Hiramatsu Akiko | Tsubaki | ep 48 |
| Kasahara Hiroko | Magdalia Sayo | |
| Ohtani Ikue | Tokiji | |
| Koorogi Satomi | Sakura | |
| Kawata Taeko | Yuki | |
| Kanemaru Junichi | Chris | |
| Hiyama Nobuyuki | Ohkuma Daigorou | |
| Iwao Junko | Yukishiro Tomoe | |
| Koyasu Takehito | Jinpuu | |
| Yuuki Hiro | Reisui | |
| Shioya Yoku | Dr. Gensai | |
| Sawaki Ikuya | Kamiya Koshijirou | |
| Komura Tetsuo | Hiruma Gohei | |
| Takemoto Eiji | Saburou | |
| Naito Rei | Sosukei | |
| Murayama Akihisa | Genta | |
| Matsuno Taiki | Nishiwaki | |
| Mori Kunihisa | Police officer | |
| Shimamoto Sumi | Tae | |
| Sakaguchi Yoshisada | Shuei boss | |
| Takagi Wataru | Onizaki Gengo | |
| Tanaka Yasurou | Onizaki Tekkan | |
| Mori Kunihisa | Robber | |
| Ohbayashi Ryuuzaburou | Tsurugi Raijuta | |
| Ushiyama Shigeru | Shakuramaru | |
| Inaba Minoru | Tsukio | |
| Sawaki Ikuya | Tsuruzaemon | |
| Kataoka Tomie | Hana | |
| Yamashita Keisuke | Sakata | |
| Tsuda Kenjirou | Toramaru | |
| Sasaoka Shigezo | Senryouyama | |
| Sawa Ritsuo | Hamakaze master | |
| Nakao Yuki | Kaese | |
| Matsuno Taiki | Yasu | |
| Hashimoto Kouichi | Kanekura | |
| Tsuji Chikahachi | Raiko | |
| Mori Kunihisa | Ginji | |
| Naito Rei | Kazu | |
| Hiyama Nobuyuki | Sasaki Heihachirou | |
| Takahashi Hiroshi | Sakaki Touma | |
| Muramatsu Yasuo | Hashizume Takuma | |
| Matamura Naomi | Kikumatsu | |
| Mizutani Seiji | Yamagata Aritomo | |
| Fuchizaki Yuriko | young Sanosuke | |
| Takagi Wataru | Shindo Tatewaki | |
| Yanaka Hiroshi | Tsuoka Tsunan | |
| Itou Kazue | Shura | |
| Komada Hajime | Ginjo | |
| Ikeda Masaru | Shibumi | |
| Nakata Kazuhiro | Akamatsu Arundo | |
| Ogai Yoko | Okita Soshi | |
| Ohtsuka Akio | Udo Jinei | |
| Irie Kanako | Komagata Yumi | |
| Komura Tetsuo | Senkaku | |
| Matamura Naomi | Mishima Eiji | |
| Hara Yasuyoshi | Yukuyama Anji | |
| Kikuchi Hidehiro | Sagara Sozo | |
| Takahashi Hiroshi | Satojima Houji | |
| Fukumoto Shinichi | Sawashimojo Chou | |
| Ishikawa Masaaki | Arai Seiku | |
| Matsumoto Miwa | Arai Iori | |
| Honda Chieko | Azusa | |
| Kiuchi Hidenobu | Shiro | |
| Cha Furin | Kuro | |
| Irie Kanako | Komagata Yumi | |
| Ryuzanji Sho | Usui | |
| Nishikawa Ikuo | Saizuchi | |
| Takeuchi Junko | Kamatari | |
| Takemoto Eiji | Henya | |
| Yashima Norito | Iwanbo | |
| Okohira Shizuka | Ochika | |
| Nakao Yuki | Omasu | |
| Hiramatsu Akiko | Tsubaki | |
| Nishihara Kumiko | Mikan | |
| Hamamoto Satoshi | Yohei | |
| Kawata Taeko | Goro | |
| Akado Mayumi | Tasuke | |
| Kitamura Kouichi | Kashiwazaki Nenji | |
| Katou Tadanari | Arai Shakku | |
| Nakao Azusa | girl with letter | |
| Nishikawa Ikuo | Saizuchi | |
| Ishizuka Noboru | Fuji | |
| Hazama Michio | Ryunosuke | |
| Imai Atsushi | Shinpachi | |
| Seto Naoko | Kinu | |
| Yuuki Hiro | Tatsuyuki | |
| Kai Ryuji | Marcus Murakami | |
| Mizutani Seiji | Yamagata Aritomo | |
| Umezu Hideyuki | Inagi Ukyo | |
| Inoue Junichi | Amakusa Shougo | |
| Kasahara Hiroko | Magdalia Sayo | |
| Akado Mayumi | Lorenzo Shozo | |
| Nakamura Hidetoshi | Kaio | |
| Nakaki Ryuji | Elsten | |
| Okura Masaaki | Santo Shobu | |
| Mikimoto Hiroyuki | Nishida Hyouei | |
| Mori Norihisa | Guspal | |
| Ohtsuka Houchuu | Mutou Tokisada | |
| Takashima Masara | Tsuruyo | |
| Ohtani Ikue | Tokiji | |
| Ogata Kenichi | Ogawa Heizo (the false Battousai) | |
| Shibamoto Hiroyuki | Anzai | |
| Kikuchi Masami | Higashiyama Ryuzaburou | |
| Yagi Kousei | Katsu Kaishu | |
| Hiyama Nobuyuki | Okuma Daigoro | |
| Iwao Junko | Katsu Ikuko | |
| Matsumoto Yasunori | Okubo Tetsuma | |
| Mugihito | Shibata | |
| Jinnai Tateyuki | Dr. Hans | |
| Yokoyama Chisa | Misanagi | |
| Yamaji Kazuhiro | Meldars | |
| Nakata Jouji | Lentz | |
| Takeoka Junichi | Schneider | |
| Cha Furin | Kurojaku | |
| Nishimura Tomohiro | Kaita | |
| Nishikawa Takanori | Orochi no Ren | |
| Koyasu Takehito | Jinpu | |
| Yuuki Hiro | Reisui | |
| Oguro Kazuhiro | Saiki | |
| Inoue Kazuhiko | Shigure Takimi | Ishinshishi he no Requiem |
| Miyamura Yuko | Takatsuki Toki | Ishinshishi he no Requiem |
| Sasaki Nozumu | Takatsuki Gentatsu | Ishinshishi he no Requiem |
| Koyasu Takehito | Kajiki Sadashiro | Ishinshishi he no Requiem |
| Kosugi Juurouta | Tamono Eibin | Ishinshishi he no Requiem |
| Yamaguchi Kappei | Musashino Yasuharu | Ishinshishi he no Requiem |
| Tanaka Rie | ? | Ishinshishi he no Requiem [debut] |